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Euthasol Solution is a non-sterile solution containing pentobarbital sodium and phenytoin sodium as the active ingredients. Rhodamine B, a bluish-red fluorescent dye, is included in the formulation to help distinguish it from parenteral drugs intended for therapeutic use. Although the solution is not sterile, benzyl alcohol, a bacteriostat, is included to retard the growth of microorganisms.
Each mL contains:
- Active ingredients: 390 mg pentobarbital sodium (barbituric acid derivative), 50 mg phenytoin sodium;
- Inactive ingredients: 10% ethyl alcohol, 18% propylene glycol, 0.003688 mg rhodamine B, 2% benzyl alcohol (preservative), water for injection q.s. Sodium hydroxide and/or hydrochloric acid may be added to adjust pH.
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EUTHASOL® Euthanasia Solution (pentobarbital sodium and phenytoin sodium) contains two active ingredients that are chemically compatible but pharmacologically different. Each ingredient acts in such a manner to cause humane, painless, and rapid euthanasia. Euthanasia is due to cerebral death in conjunction with respiratory arrest and circulatory collapse. Cerebral death occurs before cessation of cardiac activity.
When administered intravenously, pentobarbital sodium produces rapid anesthetic action. There is a smooth and rapid onset of unconsciousness. At the lethal dose, there is depression in vital medullary respiratory and vasomotor centers.
When administered intravenously, phenytoin sodium produces toxic signs of cardiovascular collapse and/or central nervous system depression. Hypotension occurs when the drug is administered rapidly.
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The sequence of events leading to humane, painless, and rapid euthanasia following intravenous injection of EUTHASOL Euthanasia Solution is similar to that following intravenous injection of pentobarbital sodium, or other barbituric acid derivatives. Within seconds, unconsciousness is induced with the simultaneous collapse of the dog. This stage rapidly progresses to deep anesthesia with a concomitant reduction in blood pressure. A few seconds later, breathing stops, due to depression of the medullary respiratory center; encephalographic activity becomes isoelectric, indicating cerebral death; and then cardiac activity ceases.
Phenytoin sodium exerts its effect during the deep anesthesia stage caused by the pentobarbital sodium. This ingredient, due to its cardiotoxic properties, hastens the stoppage of electrical activity in the heart.
Dosage: Dogs, 1 mL for every 10 pounds of body weight.
Intravenous injection is preferred. Intracardiac injection may be made when intravenous injection is impractical, as in a very small dog, or a comatose dog with impaired vascular functions. Good injection skill is necessary for intracardiac injection.
The calculated dose should be given in a single bolus injection.
For intravenous injection, a needle of sufficient gauge to ensure intravenous placement of the entire dose should be used.
The use of a Luer-Lok® syringe is recommended to prevent accidental exposure due to needle/syringe separation.
EUTHASOL Euthanasia Solution is available in 100 and 250-mL multiple-dose vials.
STORAGE at controlled room temperature of between 20° and 25°C (68° and 77°F), with excursions permitted between 15° to 30°C (59° to 86°F).